Journal: Cancer Biology & Therapy

Article Title: Deguelin inhibits perineural invasion in esophageal squamous cell carcinoma via targeting BDNF/TrkB axis

doi: 10.1080/15384047.2026.2644788

Figure Lengend Snippet: BDNF/TrkB regulate the abilities of cell proliferation, migration and invasion in KYSE-150 cells. (A and B) CCK-8 assay checked cell proliferation affected by hrBDNF and k252a; (C and D) plate cloning assay analyzed cloning formation ability of KYSE-150 cell affected by hrBDNF and k252a; (E–H) the abilities of cell invasion and migration affected by hrBDNF and k252a evaluated by scratch wound healing assays and Boyden chamber migration and invasion assays; and (I) the expression of MMP9, N-cadherin, S100B, and NGF affected by hBDNF and k252a with WB. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: Recombined human BDNF protein (Cat#450-02), k252a (Cat#HY-N6732), and Deguelin (Cat#HY-13425) were obtained from PeproTech and Med Chem Express (MCE), respectively.

Techniques: Migration, CCK-8 Assay, Cloning, Expressing

Journal: Cancer Biology & Therapy

Article Title: Deguelin inhibits perineural invasion in esophageal squamous cell carcinoma via targeting BDNF/TrkB axis

doi: 10.1080/15384047.2026.2644788

Figure Lengend Snippet: BDNF/TrkB regulate the interaction between DRG and KYSE-150 cells. (A–C) The distance of ESCC cell migration and the numbers of newborn ganglion synapses in the DRG-ESCC cells co-culture model treated with BDNF and k252a, respectively. Scale bar: 100 μM; green arrow: newborn ganglion synapses; yellow arrow: KYSE-150 cell. *** p < 0.001.

Article Snippet: Recombined human BDNF protein (Cat#450-02), k252a (Cat#HY-N6732), and Deguelin (Cat#HY-13425) were obtained from PeproTech and Med Chem Express (MCE), respectively.

Techniques: Migration, Co-Culture Assay

Journal: Cancer Biology & Therapy

Article Title: Deguelin inhibits perineural invasion in esophageal squamous cell carcinoma via targeting BDNF/TrkB axis

doi: 10.1080/15384047.2026.2644788

Figure Lengend Snippet: The role of BDNF/TrkB in ESCC PNI development in vivo . (A) The experimental flowchart of the PNI model treated with 500 μg/kg and 750 μg/kg k252a ( n = 5/group); (B and C) representative images and quantitative analysis of PNI development assessed with IVIS Spectrum in vivo imaging system; (D) representative images and quantitative analysis of the xenograft tumor in the PNI models treated with k252a; (E) representative images of the PNI model checking with HE and IHC with S100B and SCCA1; (F) The occurrence rate of PNI treated with k252a; (G–I) WB analysis and quantitative analysis of the protein expression of N-cadherin, MMP9, NGF and S100B during PNI development (40-, 50-, 60-d) and in the PNI models of the control and k252a-treated groups; (J) WB and quantitative analysis of the expression of BDNF and TrkB during PNI progress with CDX and PNI models. Red arrow: ESCC cells; black arrow: neuron; scale bar: 50 μM; * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: Recombined human BDNF protein (Cat#450-02), k252a (Cat#HY-N6732), and Deguelin (Cat#HY-13425) were obtained from PeproTech and Med Chem Express (MCE), respectively.

Techniques: In Vivo, In Vivo Imaging, Expressing, Control

Journal: Cancer Biology & Therapy

Article Title: Deguelin inhibits perineural invasion in esophageal squamous cell carcinoma via targeting BDNF/TrkB axis

doi: 10.1080/15384047.2026.2644788

Figure Lengend Snippet: BDNF/TrkB regulated ESCC PNI through Akt signaling. (A) Representative images and fluorescence intensity of the PNI model treated with k252a using IVIS spectrum in vivo imaging system ( n = 3/group); (B) representative images and quantitative analysis of the xenograft tumor in PNI models treated with k252a; (C) the clustering diagram of differentially expressed genes (DEGs) analyzed by transcriptome sequencing with xenograft tumor tissues from the k252a·PNI groups and PNI groups; (D) pathway analysis of downregulated DEGs using the GO database; (E and F) the mRNA expression levels of Akt in the PNI and k252a·PNI groups, CDX, and PNI groups using qRT-PCR; and (G–J) WB and quantitative analysis of the protein expression levels of Akt and pAkt in the PNI and k252a·PNI groups and the CDX and PNI groups at 40-, 50-, and 60-d. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: Recombined human BDNF protein (Cat#450-02), k252a (Cat#HY-N6732), and Deguelin (Cat#HY-13425) were obtained from PeproTech and Med Chem Express (MCE), respectively.

Techniques: Fluorescence, In Vivo Imaging, Sequencing, Expressing, Quantitative RT-PCR